Extraction and identification of active ingredients in Rhubarb plant

Document Type : Original Article

Author

Cereal Research Department, Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, IRAN

10.22049/cic.2023.28343.1031

Abstract

Rhubarb is a plant from the Polygonaceae family, native to Asia (likely Siberia or the Himalayas). In this study, Rheum plants were dried in a cool and dark place, and then the stem and flower powders were extracted using hexane, chloroform, and methanol solvents at room temperature with a Soxhlet apparatus. Column and plate chromatography were then used for the main separation of the extract components. The hexane, chloroform, and methanol extracts of the Rheum stem were separated into 11, 8, and 7 parts, respectively, using the solvent systems of ethyl acetate/petroleum ether, chloroform/methanol/ethyl acetate, and methanol/ethyl acetate/water/acetic acid. The pure compounds were identified using FT-IR, H1 NMR, and C13 NMR spectroscopy.

Keywords

References

[1] Zghonda N, Yoshida S, Ezaki S, Otake Y, Murakami C, Mliki A, Bioscience, biotechnology, and biochemistry, 2012, 954-60
[2] Sitasawad SL, Shewade Y, Bhonde R. Journal of Ethnopharmacology, 2000, 71-9
[3] Aziz A, Khaliq T, Khan JA, Jamil A, Majeed W, Faisal MN, Pakistan Journal of Agricultural Sciences, 2017
[4] Ahmed SR, Rabbee MF, Roy A, Chowdhury R, Banik A, Kubra K, Plants, 2021, 1348
[5] Nirmala C, Shahar B, Dolma N, Santosh O. Applied Food Research, 2019
[6] Cai Y, Sun M, Xing J, Corke H. Journal of agricultural and food chemistry, 2004, 7884-90.
[7] Stanaway JD, Afshin A, Gakidou E, Lim SS, Abate D, Abate KH, 2018, 1923-94.
[8] Öztürk M, Aydoğmuş-Öztürk F, Duru ME, Topçu G. Food chemistry, 2007, 623-30.
[9] Ahmad A, Patel I. J Pharm Bioallied Sci, 2013, 326.
[10] Haggag E, Mahmoud I, Abou-Moustafa E, Mabry T. Asian Journal of Chemistry, 1999, 707-14.
[11] El-Hilaly J, Tahraoui A, Israili ZH, Lyoussi B. Pak J Pharm Sci. 2007, 261-8.
[12] Alenzi F, El-Bolkiny YE-S, Salem M. British journal of biomedical science, 2010, 20-8.
[13] Sousa PL, Quinet Y, Ponte EL, do Vale JF, Torres AFC, Pereira MG, Journal of Ethnopharmacology, 2012, 213-6.
[14] Agarwal S, Singh SS, Verma S, Kumar S. Journal of ethnopharmacology, 2000, 43-6.
[15] AKHTAR MS, QAYYUM MI, IRSHAD N, HUSSAIN R, HUSSAIN A, Yaseen M, Int J Curr Pharm Res, 2013, 36-7.
[16] Zargar BA, Masoodi MH, Ahmed B, Ganie SA. Food Chemistry, 2011, 585-9.
[17] He Z-H, He M-F, Ma S-C, But PP-H. Journal of ethnopharmacology, 2009, 313-7.
[18] Uddin G, Rauf A, Siddiqui BS, Shah SQ. Middle-East Journal of Scientific Research, 2011, 78-81.
[19] Patel V, Patel R. Journal of Chemical and Pharmaceutical Research, 2016, 1423-43.
[20] Aburjai TA. Phytochemistry, 2000, 407-10.
 

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History

  • Receive Date: 22 February 2023
  • Accept Date: 17 March 2023

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